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Under such overcrowded living conditions poor sanitation and hygiene were likely and individuals no doubt had limited access to safe drinking water, contributing to increased potential for infectious disease outbreaks [].

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A total of 1,845 blood donors eligible to donate blood as per the criteria of the Central Blood Transfusion Service (CBTS), Nepal Red Cross Society (NRCS) were included in this study.All samples were also tested for HEV-Ig G (Wantai HEV Ig G ELISA, Beijing Wantai Biological Pharmacy Enterprise Co., Ltd) and HEV antigen (Wantai HEV-Ag Donor data obtained from the questionnaire were entered in to a Microsoft Excel 2010 (Redmond, WA, USA) database.Proportions of donors HEV Ig G, Ig M or antigen positive were calculated overall and for each of the study variables, and 95% confidence intervals estimated.The proportion of donors with both HEV Ig M and Ig G was 2.7%.HEV Ig M prevalence was associated with a donor reporting having a history of jaundice or reporting pork consumption ().Recent devastating earthquakes in Nepal could have facilitated an outbreak of HEV.

In this study, we measured previous and current HEV infection in Nepalese blood donors after recent major earthquakes to provide surveillance data on HEV in Nepal and to determine possible risk factors for HEV exposure.

Overall, 41.9% (773/1,845; 95% CI 39.7–44.2%) of donors were HEV Ig G positive, with regional variation observed.

Higher HEV Ig G and Ig M prevalence was observed in donors who reported eating pork, likely an indicator of zoonotic transmission.

Samples were tested for HEV Ig M, Ig G and antigen.

The proportion of donors positive for HEV Ig M or Ig G was calculated overall, and for each of the variables studied.

= 1,845) were collected from blood donors from Kathmandu, Chitwan, Bhaktapur and Kavre.