Relaxed phylogenetics and dating with confidence
umbellatus showed a high diversity and belonged to five independent phylogenetic lineages, including lineage 6, lineage 4, lineage 8, lineage 1, and A. Twenty-three Armillaria isolates, i.e., almost half of the total isolates, belonged to lineage 6. Some isolates present evident geographic characteristics. Isolates from Shanxi, Gansu, Henan and Hebei provinces were all identified with lineage 6.Isolates from Northeast China, such as Jilin and Heilongjiang, belonged to A.
The red labels on the nodes of the phylogram indicate the new lineage (lineage 8) recognized in this research. The values of the bootstrap frequencies of ML (BP The Armillaria isolates associated with P. Relative abundances of phylogenetic lineages of Armillaria isolates are shown in Fig. One singleton (M3) showed relatively long branches compared with its sister group (Fig. At present, the M3 strain is considered to be genetically divergent from its sisters.umbellatus, there have been few studies on the association of Armillaria species with P. Among the four matrices, ITS phylogeny (Supplementary Information, Figure S1C) and three-locus phylogeny (Fig.1) present the lowest and the best branch resolution and support, respectively.umbellatus impose structure on the interaction matrix. In China, 16 CBS of Armillaria were identified by mating tests.However, due to the limits of mating tests, these CBS still need further verification via by modern molecular approaches. effectively established the link between the CBS and the phylogenetic lineages and identified at least 15 phylogenetic lineages in China.This lineage was defined as a new phylogenetic lineage in Guo et al..
This lineage is represented in most of the samples from China previously considered as A. Additionally, the Armillaria species used in the cultivation of P. This study also found that the Armillaria isolates present certain geographic characteristics.
gallica, which was strongly divergent from European A. However, we also found evident divergence in lineage 6 which formed two subgroups in the β-tubulin phylogeny (Supplementary Information, Figure S1A). However, there has been no evidence to date to suggest that A. For example, Armillaria isolates from Northeast China are mainly A.
In contrast, ML bootstrap for this lineage was low in the EF-1α phylogeny (. umbellatus associates with diverse Armillaria partners. Previously published articles named the Armillaria species with which P. cepistipes, while isolates from Southwest China mainly belong to lineage 4.
umbellatus populations tend to interact with phylogenetically related Armillaria species.
Phylogenetic network analyses revealed that the interaction between P.
Only a few branches were supported by bootstrap and posterior probabilities for the ITS phylogeny.